50.8 Active B12

50.8.1 Global tests

Type III ANOVA on main and interaction effects, and LM model summaries.

PRL (ng/ml)
Chisq Df Pr(>Chisq)
(Intercept) 67.4457733 1 <0.0001 ***
Active.B12 0.1176992 1 0.7 ns
Study 0.1615703 1 0.7 ns
GA_samp 58.6983233 1 <0.0001 ***
Active.B12:Study 0.0319285 1 0.9 ns
hPL (μg/ml)
Chisq Df Pr(>Chisq)
(Intercept) 6.1178575 1 0.01 *
Active.B12 0.0010379 1 1.0 ns
Study 3.0037869 1 0.08 ns
GA_samp 832.2474984 1 <0.0001 ***
Active.B12:Study 0.0508695 1 0.8 ns
GH2 (ng/ml)
Chisq Df Pr(>Chisq)
(Intercept) 193.194888 1 <0.0001 ***
Active.B12 1.660906 1 0.2 ns
Study 2.898371 1 0.09 ns
GA_samp 463.652179 1 <0.0001 ***
Active.B12:Study 2.181948 1 0.1 ns
Insulin (uU/ml)
model term df1 df2 F.ratio Chisq p.value
1 Active.B12 1 Inf 0.122 0.122 0.7 ns
3 Study 1 Inf 2.404 2.404 0.1 ns
4 GA_samp 1 Inf 0.098 0.098 0.8 ns
2 Active.B12:Study 1 Inf 0.008 0.008 0.9 ns
HOMA-IR
model term df1 df2 F.ratio Chisq p.value
1 Active.B12 1 Inf 0.136 0.136 0.7 ns
3 Study 1 Inf 4.369 4.369 0.04 *
4 GA_samp 1 Inf 0.177 0.177 0.7 ns
2 Active.B12:Study 1 Inf 0.023 0.023 0.9 ns
Glucose (mmol/L)
model term df1 df2 F.ratio Chisq p.value
1 Active.B12 1 Inf 1.951 1.951 0.2 ns
3 Study 1 Inf 11.796 11.796 0.0006 ***
4 GA_samp 1 Inf 0.769 0.769 0.4 ns
2 Active.B12:Study 1 Inf 0.057 0.057 0.8 ns

50.8.2 Model summary

Ratio of Geometric means (95% CI)

PRL (ng/ml)
Var Effect P
(Intercept) 14.66 (7.87, 27.29) <0.0001
Active.B12 1.00 (1.00, 1.00) 0.75
StudySTOP 0.91 (0.76, 1.10) 0.34
GA_samp 1.16 (1.11, 1.20) <0.0001
hPL (μg/ml)
Var Effect P
(Intercept) 1.53 (1.09, 2.15) 0.01
Active.B12 1.00 (1.00, 1.00) 0.75
StudySTOP 1.19 (1.08, 1.32) 0.0008
GA_samp 1.36 (1.33, 1.38) <0.0001
GH2 (ng/ml)
Var Effect P
(Intercept) 0.05 (0.03, 0.07) <0.0001
Active.B12 1.00 (1.00, 1.00) 0.81
StudySTOP 1.06 (0.93, 1.20) 0.39
GA_samp 1.33 (1.30, 1.37) <0.0001
Insulin (uU/ml)
Var Effect P
(Intercept) 16.87 (7.76, 36.71) <0.0001
Active.B12 1.00 (1.00, 1.00) 0.74
StudySTOP 0.71 (0.57, 0.90) 0.004
GA_samp 0.99 (0.95, 1.04) 0.75
HOMA-IR
Var Effect P
(Intercept) 4.28 (1.88, 9.74) 0.0005
Active.B12 1.00 (1.00, 1.00) 0.73
StudySTOP 0.62 (0.48, 0.79) 0.0001
GA_samp 0.99 (0.94, 1.04) 0.67
Glucose (mmol/L)
Var Effect P
(Intercept) 5.66 (4.96, 6.46) <0.0001
Active.B12 1.00 (1.00, 1.00) 0.16
StudySTOP 0.86 (0.83, 0.90) <0.0001
GA_samp 1.00 (0.99, 1.01) 0.38

50.8.3 Estimated means

Estimated marginal means (or adjusted means) adjusted for GA at sampling.

Results are averaged over the levels of Active B12 concentration (pM) and may be misleading when interaction term is involved.

PRL (ng/ml)
Study response SE df lower.CL upper.CL
SCOPE 104.01066 9.614865 132.9306 86.63012 124.8783
STOP 94.94685 8.201065 102.8474 79.99860 112.6883
hPL (μg/ml)
Study response SE df lower.CL upper.CL
SCOPE 90.59169 3.234625 260.1075 84.44106 97.19032
STOP 107.89007 3.433358 166.1723 101.32000 114.88617
GH2 (ng/ml)
Study response SE df lower.CL upper.CL
SCOPE 2.293938 0.0976149 313.1236 2.109694 2.494272
STOP 2.425388 0.0890781 204.3571 2.255967 2.607534
Insulin (uU/ml)
Study response SE df asymp.LCL asymp.UCL
SCOPE 14.72221 1.1449052 Inf 12.640885 17.14623
STOP 10.51991 0.6520471 Inf 9.316497 11.87877
HOMA-IR
Study response SE df asymp.LCL asymp.UCL
SCOPE 3.563678 0.2995429 Inf 3.022395 4.201900
STOP 2.196609 0.1440558 Inf 1.931657 2.497902
Glucose (mmol/L)
Study response SE df asymp.LCL asymp.UCL
SCOPE 5.538397 0.0830353 Inf 5.378018 5.703558
STOP 4.767779 0.0589751 Inf 4.653580 4.884780

50.8.4 Comparisons

Effect for every 10 unit increase in Active B12 concentration (pM).
Hormone Contrast Study Ratio of Geometric means (95% CI) P-value
PRL (ng/ml) Active.B1270 / Active.B1260 SCOPE 1.00 (0.98, 1.03) 0.7
PRL (ng/ml) Active.B1270 / Active.B1260 STOP 1.00 (0.98, 1.03) 0.7
hPL (μg/ml) Active.B1270 / Active.B1260 SCOPE 1.00 (0.99, 1.01) 0.7
hPL (μg/ml) Active.B1270 / Active.B1260 STOP 1.00 (0.99, 1.01) 0.7
GH2 (ng/ml) Active.B1270 / Active.B1260 SCOPE 1.00 (0.99, 1.02) 0.8
GH2 (ng/ml) Active.B1270 / Active.B1260 STOP 1.00 (0.99, 1.02) 0.8
Insulin (uU/ml) Active.B1270 / Active.B1260 SCOPE 0.99 (0.96, 1.03) 0.7
Insulin (uU/ml) Active.B1270 / Active.B1260 STOP 0.99 (0.96, 1.03) 0.7
HOMA-IR Active.B1270 / Active.B1260 SCOPE 0.99 (0.96, 1.03) 0.7
HOMA-IR Active.B1270 / Active.B1260 STOP 0.99 (0.96, 1.03) 0.7
Glucose (mmol/L) Active.B1270 / Active.B1260 SCOPE 1.01 (1.00, 1.01) 0.2
Glucose (mmol/L) Active.B1270 / Active.B1260 STOP 1.01 (1.00, 1.01) 0.2

50.8.5 Diagnostics

Model diagnositcs.

Diagnositcs for PRL (ng/ml)

Diagnositcs for hPL (μg/ml)

Diagnositcs for GH2 (ng/ml)

Diagnositcs for Insulin (uU/ml)

Diagnositcs for HOMA-IR

Diagnositcs for Glucose (mmol/L)

50.8.6 Plots